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ELISA is an acronym that stands for enzyme-linked immunosorbent assay. This technology is also sometimes referred to as EIA which stands for enzyme immunoassay.

ELISAs are a commonly used analytical technique to detect and quantify an analyte in a sample using antibodies that have highly specific binding affinities for the analyte.

In ELISA methodology, two essential components for quantification are highly specific antibodies that bind to their target and the immobilization of the analyte. The analyte or antibody is immobilized by attaching it to a surface, typically a 96-well microtiter plate or test tube, using a passive binding process. Quantification is achieved by using a purified analyte or antibody that is conjugated to an enzyme, often horse radish peroxidase (HRP), which binds to the immobilized target. Any non-specific interactions are eliminated by washing the surface. The final step involves exposing the enzyme to its substrate, resulting in a detectable signal, usually a color change. To halt the conversion of substrate to product, a solution may be added after a specific period of time. The signal from the sample is then compared to a standard curve to determine the concentration of the antigen present in the sample.


Plate Assay Video

Watch the video below to learn step-by-step instructions on how to run our Aflatoxin Plate Kit and successfully analyze your samples. While this video is assay-specific, the techniques described in the video remain the same across all of our plate assays. 

Tube Assay Video

View the video below for a comprehensive guide on running our Aflatoxin Tube Kit, providing you with the necessary instructions to perform accurate sample analysis. Although this video focuses on a specific assay, the techniques demonstrated are applicable to all of our tube assays.


Access the file below to view our ELISA Troubleshooting Guide which will walk you through potential sources of error in immunoassay methodology as well as proper assay techniques.


All manufacturing operations done at Beacon are conducted in compliance with GMP (Good Manufacturing Practices) regulations.


Beacon Analytical  Systems, Inc. ("Beacon") warrants the products manufactured by it against defects in materials and workmanship when used in accordance with the applicable instructions for a period not to extend beyond a product's printed expiration date. BEACON MAKES NO OTHER WARRANTY, EXPRESSED OR IMPLIED. THERE IS NO WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. The warranty provided herein and the data, specifications, and descriptions of Beacon products appearing in published catalogs and product literature may not be altered except by an express written agreement signed by an officer of Beacon. Representations, oral or written, which are inconsistent with this warranty or such publications are not authorized and, if given, should not be relied upon. 


In the event of a breach of the foregoing warranty, Beacon's sole obligation shall be to repair or replace, at its option, any product or part thereof that proves defective in materials or workmanship within the warranty period, provided the customer notifies Beacon promptly of any such defect. The exclusive remedy provided herein shall not be deemed to have failed of its essential purpose so long as Beacon is willing and able to repair or replace any nonconforming Beacon product or part. Beacon shall not be liable for consequential, incidental, special or any other indirect damages resulting from economic loss or property damage sustained by a customer from the use of its products. However, in some states, the purchaser may have rights under state law in addition to those provided by this warranty. 

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